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毛冠鹿种内异染色质变化与染色体多态
引用本文:粘伟红,庞宏,张锡然,曹祥荣,刘伟,王强.毛冠鹿种内异染色质变化与染色体多态[J].动物学杂志,2006,41(6):132-135.
作者姓名:粘伟红  庞宏  张锡然  曹祥荣  刘伟  王强
作者单位:1. 南京师范大学江苏省分子医学生物技术重点实验室,南京,210097
2. 四川省成都动物园,成都,610081
基金项目:国家自然科学基金(No.30370789),江苏省教委自然科学基金(No.02KJD180006)
摘    要:采用原代和传代培养方法对8头毛冠鹿(Elaphodus cephalophus)的皮肤细胞进行了染色体研究,发现了一种核型与以前所报道的几种核型不一致,确定为一新核型。在该核型中,染色体众数2n=47,2条X染色体异型,一条为端着丝粒,另一条为近端着丝粒。C-带显示该核型中异染色质除了分布在2条X染色体长臂中之外,在第一对大的端着丝粒染色体中的一条近着丝粒区出现一异染色质“柄”。结合C-带及薄层扫描结果对毛冠鹿种内常染色体、性染色体中异染色质的含量和分布与染色体多态的关系进行了探讨。

关 键 词:异染色质  染色体多态  核型  C-显带  薄层扫描
文章编号:0250-3263(2006)06-132-04
收稿时间:2006-03-30
修稿时间:2006-03-302006-09-15

Variation of Heterochromatin and Chromosome Polymorphism in the Tufted Deer
NIAN Wei-Hong,PANG Hong,ZHANG Xi-Ran,CAO Xiang-Rong,LIU Wei,WANG Qiang.Variation of Heterochromatin and Chromosome Polymorphism in the Tufted Deer[J].Chinese Journal of Zoology,2006,41(6):132-135.
Authors:NIAN Wei-Hong  PANG Hong  ZHANG Xi-Ran  CAO Xiang-Rong  LIU Wei  WANG Qiang
Institution:2 The Jiangsu Key Laboratory of Molecular and Medical Biotechnology of Nanjing Normal University, Nanjing 210097; 2 Chengdu Zoo , Chengdu 610081,China
Abstract:Chromosomal analysis of skin cells from 8 Tufted Deer(Elaphodus cephalophus) was conducted by using primary culture and subculture.A new karyotype,different from the previously reported karyotype,was found.There were 47 chromosomes in somatic cells,including a pair of heteromorphic X chromosomes,one was telocentric and the other was subtelocentric.C-banding revealed that besides the two long arms of the X chromosomes the heterochromatin also displayed in the area near the centric region of one of the first telocentric chromosome,looking like an "arm".We also studied the relationship between the quantity and display of heterochromatin in the Tufted Deer's autosomes and sex chromosomes and the chromosome polymorphism according to the results of C-banding and thin layer scanning.
Keywords:Heterochromatin  Chromosome polymorphism  Karyotype  C-banding  Thin layer scanning
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