The development of mouse spinal cord in tissue culture |
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Authors: | B H J Juurlink S Fedorff |
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Institution: | (1) Department of Anatomy, University of Saskatchewan, S7N 0W0 Saskatton, Saskatchewan, Canada |
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Abstract: | Summary Neural tubes of mouse embryos at Theiler Stages 14, 15, and 16 were grown in cultures for 21 d with 0.5 μCi/ml tritiated thymidine
or cold growth medium. It was found that 50 to 60% of the neurons formed in the outgrowth zone were labeled, indicating that
they formed from precursor cells that proliferated in the cultures. The unlabeled neurons must have formed from cells that
were already postmitotic when the cultures were started. By comparing the total number of neurons per neuromere formed in
vivo and in vitro, it seems that the postmitotic precursor cells survive better in cultures and only a small percentage of
proliferative precursor cells in cultures enter the postmitotic stage and form neurons.
This work was supported by Grant MT4235 from the Medical Research Council of Canada. |
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Keywords: | neural tube neuronogenesis mouse precusor cells neurons |
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