| 针叶松遗传转化操作的研究进展(英文) |
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| 引用本文： | 唐巍,Ross Whetten,Ron Sederoff. 针叶松遗传转化操作的研究进展(英文)[J]. 基因组蛋白质组与生物信息学报(英文版), 2001, 0(1) |
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| 作者姓名： | 唐巍 Ross Whetten Ron Sederoff |
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| 作者单位： | 美国NC27695-7247北卡罗林那州立大学森林系森林生物技术室，美国NC27695-7247北卡罗林那州立大学森林系森林生物技术室，美国NC27695-7247北卡罗林那州立大学森林系森林生物技术室 |
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| 摘 要： | 1986年从火炬松方面获得了首例针叶松愈伤组织以后，针叶松遗传转化研究取得了明显的进展。1991年从Larix decidua方面获得了第一个转基因再生植株。最近，通过农瘤杆菌介导的转化方式在Larix kaempferi ( L. Decidua杂交树种上获得了转基因针叶松，通过鸟枪法在Picea glauca、Picea mariana、Picea abies、Larix laricina、Pinus radiata上亦获得了转基因植株。已经有多种办法用于转化针叶松。本文就目前在针叶松上所用的遗传转化方法??诸如：T－DNA转化法、农瘤杆菌介导转法化、鸟枪法、电转化法等给以粗略的评论。
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Advances on Genetic Transformation in Conifers |
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| TANG Wei,Ross Whetten and Ron Sederoff Forest Biotechnology Group. Advances on Genetic Transformation in Conifers[J]. Genomics, proteomics & bioinformatics, 2001, 0(1) |
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| Authors: | TANG Wei Ross Whetten Ron Sederoff Forest Biotechnology Group |
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| Affiliation: | TANG Wei1,Ross Whetten and Ron Sederoff Forest Biotechnology Group,Department of Forestry,North Carolina State University,Raleigh,NC 27695-7247,USA |
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| Abstract: | Considerable progress has been made in genetic transformation in conifers since the first conifer tumor was produced in 1986 from loblolly pine[1]. The first transgenic regenerated plants in conifers were obtained from Larix decidua in 1991[2]. Recently, transgenic conifers have been produced from hybrid larch (Larix kaempferi ( L. decidua) via Agrobacterium-mediated transformation[3], and from Picea glauca[4], Picea mariana[5], Picea abies[6,7], Larix laricina[8],and Pinus radiata[9] by particle bombardment. Different transformation methods have been widely employed to transform conifers. In this review, we provide a brief overview of the current state of knowledge on genetic transformation in conifers involved in T-DNA transfer process, Agrobacterium-mediated transformation, transformation via particle bombardment, and transformation using electroporation. |
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| Keywords: | genetic transformation conifers Agrobacterium biolistic electroporation |
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