Transformation of the basidiomycete, Schizophyllum commune |
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| Authors: | Alfredo Munoz-Rivas Charles A Specht Bruce J Drummond Eunice Froeliger Charles P Novotny and Robert C Ullrich |
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| Institution: | (1) Department of Botany, University of Vermont, 05405 Burlington, Vermont, USA;(2) Department of Medical Microbiology, University of Vermont, 05405 Burlington, Vermont, USA |
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| Abstract: | Summary Protoplasts of aSchizophyllum commune tryptophan auxotroph (trp1), deficient in indole-3-glycerol phosphate synthetase (IGPS), were transformed to trp+ with plasmid DNA containing the SchizophyllumTRP1 sequence. Efficiencies up to 30 transformants per microgram of plasmid DNA were obtained. Southern blots reveal that the
transforming DNA is integrated in chromosomal DNA. The trp+ phenotype of transformants is stable in meiosis and mitosis. Transformants possess IGPS activity comparable to wild-type
cells. |
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| Keywords: | Schizophyllum Basidiomycete Transformation tryptophan |
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