A generic protein purification method for protein complex characterization and proteome exploration. |
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Authors: | G Rigaut A Shevchenko B Rutz M Wilm M Mann B Séraphin |
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Affiliation: | European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. |
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Abstract: | We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms. |
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