Indigo and indirubin derivatives from indoles in Polygonum tinctorium tissue cultures |
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Authors: | Jae-Yoon Shim Yung-Jin Chang Soo-Un Kim |
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Institution: | (1) Department of Agricultural Chemistry and the Research Center for New Biomaterials in Agriculture, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea |
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Abstract: | Substituted indoles (5-bromo-, 5-fluoro-, 5-chloro-, 5-hydroxy-, 4-methyl-, 5-methyl-, 6-methyl-, and 7-methylindole) were fed at 1 mM for 10 to 21 days into the tissue cultures of Polygonum tinctorium including cell suspension, root and shoot cultures. They were converted into the corresponding indirubin and indigo derivatives with the exception of 5-hydroxyindole. The yield ranged between 0.7–1.1 mg/g dried weight for indigos and 0.007–0.024 mg/g dried weight for indirubins. Presence of the corresponding indican derivatives in the shoot culture suggested that the pigment production proceeded through the indican formation. © Rapid Science Ltd. 1998 |
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Keywords: | indigo indirubin plant tissue culture Polygonum tinctorium |
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