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Characteristics of Ralstonia solanacearum Biovar N2 Strains in Asia
Authors:M. Horita    K. Tsuchiya    A. Ooshiro
Affiliation:Authors' addresses: National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555, Japan;;National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan;;Okinawa Prefectural Agricultural Experiment Station, Naha, Okinawa 903-0814, Japan (correspondence to M. Horita. E-mail: )
Abstract:Ralstonia solanacearum biovar N2 strains isolated in Asia were compared by biochemical tests with biovar N2 strains from South America and biovar 2 (race 3) strains from Africa, America, Asia and Europe. Distinct differences were found between Asian and South American strains of biovar N2, and between Asian biovar N2 and biovar 2 strains with respect to their ability to utilize several carbon sources. Using cluster analysis based on repetitive sequence‐based polymerase chain reaction (rep‐PCR) genomic fingerprints, the Asian biovar N2 strains were divided into two groups, group 1 containing Japanese strains and group 2 containing Indonesian and Philippine strains. The fingerprints showed the genetic diversity of biovar N2 strains in Asia.
Keywords:Ralstonia solanacearum    Asian strains    biovar N2    repetitive sequence-based polymerase chain reaction
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