Androstenedione metabolism in human lung fibroblasts |
| |
Authors: | L Milewich V Kaimal C B Shaw A R Johnson |
| |
Institution: | 1. Department of Rheumatology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands;2. Struttura Complessa di Reumatologia, Rheumatology Unit, Azienda Policlinico of Modena, University of Modena and Reggio Emilia, Largo del Pozzo 71, 41125 Modena, Italy;3. Medicine and Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, via Pupilli 1, 40136 Bologna, Italy;4. Walaeus Library, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands |
| |
Abstract: | Human lung fibroblasts in culture metabolized 3H]androstenedione to a number of different compounds, including testosterone, 5 alpha-androstanedione, androsterone, 5 alpha-dihydrotestosterone, isoandrosterone, and 5 alpha-androstane-3 alpha,-17 beta-diol. The major products were 5 alpha-androstanedione and testosterone. Estrone, estradiol-17 beta and 5 beta-reduced steroids were not formed. The production rates of testosterone and 5 alpha-androstanedione from 3H]androstenedione by lung fibroblasts were studied both as a function of incubation time and substrate concentration. The rates of formation of testosterone and 5 alpha-androstanedione remained linear with time up to 4 h. The apparent Km of human lung fibroblast 5 alpha-reductase was 1 microM, and that of 17 beta-hydroxysteroid oxidoreductase was 11 microM. The findings of this study suggest that mesenchyma may contribute to the metabolism of androstenedione in human lung tissue. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|