Cation shifts in human retina-choroid |
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Authors: | Barbara J Panessa-Warren Hobart W Kraner Keith W Jones Cynthia Kaplan |
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Institution: | (1) Allied Health Resources, Health Sciences Center, State University of New York, Stony Brook, 11794 Stony Brook, NY;(2) Instrumentation Division, Brookhaven National Laboratory, 11973 Upton, NY;(3) Department of Applied Science, Brookhaven National Laboratory, 11973 Upton, NY;(4) Department of Pathology, Health Sciences Center, State University of New York, Stony Brook, 11794 Stony Brook, NY |
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Abstract: | Human ocular tissues from 50 donor eyes were elementally and morphologically analyzed in order to correlate the elemental
content and distribution of Ca, Ba, Cr, Cu, Zn, and Se with ocular morphology, sex, race, irideal pigmentation, age, time
of death, birth weight, presence and severity of diabetes, and other pathologies noted at autopsy. Initially, to facilitate
the transport of donor tissue to the laboratory, the eyes were fixed in glutaraldehyde. Because our preliminary data revealed
alterations in elemental content following chemical fixation of ocular tissues, all of the subsequent samples were analyzed
in their fresh, hydrated (unfixed) condition as soon after enucleation as possible. Samples were elementally analyzed by X-ray
fluorescence spectrometry (XRF) and proton-induced X-ray emission spectrometry (PIXE) using high resolution Si(Li) X-ray detectors.
Tissue was morphologiscally examined by scanning electron microscopy and light microscopy histoichemistry. |
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Keywords: | Proton-induced X-ray emission spectrometry human ocular tissue X-ray fluorescence spectrometry Ca Ba Cr Zn Cu Se analysis diabetic retina-choroid fetal retina-choroid and pediatric retina-choroid |
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