| 糖尿病性高血压大鼠肾损害相关基因cDNA片段的克隆与初步鉴定 |
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| 引用本文： | 张芳林,李果,叶传忠,丁伟,刘优萍,谢超,张迪,孙卫华,罗敏. 糖尿病性高血压大鼠肾损害相关基因cDNA片段的克隆与初步鉴定[J]. 生物化学与生物物理进展, 2002, 29(1): 94-99 |
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| 作者姓名： | 张芳林 李果 叶传忠 丁伟 刘优萍 谢超 张迪 孙卫华 罗敏 |
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| 作者单位： | 1. 上海第二医科大学附属瑞金医院,上海市内分泌研究所,上海,200025
2. 复旦大学附属中山医院泌尿外科,上海,200032 |
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| 摘 要： | 为筛选大鼠糖尿病性高血压病所致的肾损害相关基因，自发性高血压大鼠以STZ 50 mg/kg一次性尾静脉注射，建立糖尿病性高血压大鼠模型，4周后该模型大鼠尿蛋白持续阳性,电镜观察到肾小球基质增生、足突融合或扁平.应用荧光标记的差异显示反转录聚合酶链反应(DDRT-PCR)及RNA印迹技术，比较两种模型大鼠肾皮质的基因表达差异，并进行差异条带的cDNA序列生物信息学分析.其中4个差异条带与Genbank数据库比较，2个为未知基因，2个为已知基因，即成淀粉样糖蛋白（amyloidogenic glycoprotein,AGG）、CDK109，两者可能与糖尿病性高血压肾损害的发生相关，对它们的进一步研究将为肾损害发病机理的发现提供思路.
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| 关 键 词： | 差异显示，糖尿病性高血压，肾，生物信息学 |
| 收稿时间： | 2001-08-13 |
| 修稿时间： | 2001-08-13 |
Cloning and Identification of cDNA Fragments Related to Renal Damage of Diabetic Hypertension Rats |
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| ZHANG Fang-Lin,LI Guo,YE Chuan-Zhong,DING Wei,LIU You-Ping,XIE Chao,ZHANG Di,SUN Wei-Hua and LUO Min. Cloning and Identification of cDNA Fragments Related to Renal Damage of Diabetic Hypertension Rats[J]. Progress In Biochemistry and Biophysics, 2002, 29(1): 94-99 |
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| Authors: | ZHANG Fang-Lin LI Guo YE Chuan-Zhong DING Wei LIU You-Ping XIE Chao ZHANG Di SUN Wei-Hua LUO Min |
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| Affiliation: | Shanghai Institute of Endrocrinology, Ruijin Hospital,Shanghai Second Medical University, Shanghai 200025, China;Shanghai Institute of Endrocrinology, Ruijin Hospital,Shanghai Second Medical University, Shanghai 200025, China;Department of Urology, the Affiliated Zhongshan Hospital, Medical Center of Fudan University, Shanghai 200032, China;Shanghai Institute of Endrocrinology, Ruijin Hospital,Shanghai Second Medical University, Shanghai 200025, China;Shanghai Institute of Endrocrinology, Ruijin Hospital,Shanghai Second Medical University, Shanghai 200025, China;Shanghai Institute of Endrocrinology, Ruijin Hospital,Shanghai Second Medical University, Shanghai 200025, China;Shanghai Institute of Endrocrinology, Ruijin Hospital,Shanghai Second Medical University, Shanghai 200025, China;Shanghai Institute of Endrocrinology, Ruijin Hospital,Shanghai Second Medical University, Shanghai 200025, China;Shanghai Institute of Endrocrinology, Ruijin Hospital,Shanghai Second Medical University, Shanghai 200025, China |
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| Abstract: | |
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| Keywords: | differential display diabetic hypertension kidney bioinformatics |
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