Single-step chromatography for simultaneous purification of C-phycocyanin and allophycocyanin with high purity and recovery from <Emphasis Type="Italic">Spirulina</Emphasis> (<Emphasis Type="Italic">Arthrospira</Emphasis>) <Emphasis Type="Italic">platensis</Emphasis> |
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Authors: | Shi-Gan?Yan Li-Ping?Zhu Hai-Nan?Su Xi-Ying?Zhang Xiu-Lan?Chen Bai-Cheng?Zhou Email author" target="_blank">Yu-Zhong?ZhangEmail author |
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Institution: | (1) State Key Lab of Microbial Technology, Shandong University, Jinan, 250100, China;(2) School of Food and Bioengineering, Shandong Institute of Light Industry, Jinan, 250353, China;(3) Shandong Academy of Agricultural Sciences, Jinan, 250100, China; |
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Abstract: | The cyanobacterium Spirulina (Arthrospira) platensis is a good source of phycobiliprotein purification. C-phycocyanin (C-PC) is the major phycobiliprotein, while allophycocyanin
(APC) is less abundant in S. platensis. Previously reported methods for C-PC purification are only able to offer either high purity or high efficiency. This paper
describes one-step anion exchange chromatography method with continuous pH gradient elution for simultaneous purification
of C-PC and APC with high purity and high recovery. Crude C-PC and APC were extracted and concentrated by ammonium sulfate
fractionation at saturation of 25% and 60%, then purified on a DEAE-Sepharose Fast Flow chromatography column with continuous
pH gradient elution from pH 5.0 to 3.6. After this single-step chromatography, C-PC and APC with high purity and recovery
were simultaneously obtained. The purity ratios of C-PC and APC reached 5.59 (A620/A280) and 5.19 (A650/A280), respectively. Their purity was further demonstrated by electrophoresis and fluorescence emission spectroscopy. Moreover,
the total recovery yield of pure C-PC and APC were 67.04% and 80.0%, representing 111.83 and 29.28 mg·g−1 lyophilized weight, respectively. The obtained C-PC and APC remained stable over a pH range of 4–9. This purification method
for high purity and recovery of C-PC and APC proved to be fairly efficient compared with previously reported methods. |
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