An improved cosmid vector for the cloning of equine herpesvirus DNA |
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| Authors: | Lesley Nicolson E. Linda J. Rafferty Andrena Brawley David E. Onions |
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| Affiliation: | Department of Veterinary Pathology, University of Glasgow Veterinary School, Glasgow G61 1QH, UK |
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| Abstract: | We have modified the commercial cosmid vector, triple helix vector (THV), such that I-Sce-I restriction endonuclease sites flank the cloning site. I-Sce-I is a rare-cutting endonuclease which recognizes an 18-bp sequence. It does not restrict the genome of either of the equine herpesvirus 1 or 4 (EHV-1 and EHV-4) strains we have cosmid cloned. Thus, cosmid- cloned EHV fragments can be excised intact from the vector by I-Sce-I digestion, facilitating production of large overlapping EHV fragments for use in transfections to produce recombinant virus. |
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| Keywords: | Recombinant DNA rare cutters restriction enzymes recombination |
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