木耳栽培菌株酯酶同工酶的酶谱多样性研究

采用聚丙烯酰胺凝胶电泳技术对木耳（Ａｕｒｉｃｕｌａｒｉａａｕｒｉｃｕｌａ（Ｈｏｏｋ）Ｕｎｄｅｒｗ）１０个栽培菌株酯酶同工酶的酶谱多样性进行了研究。１０个供试菌株的幼龄菌丝（７ｄ）中仅检测到２５条酶带，各个菌株分别具有２～３条酶带，１０个菌株仅有２种酶谱类型；老龄菌丝（７２ｄ）中共检测到４４条酶带，各个菌株分别具有３～６条酶带，１０菌株共有８种酶谱类型。研究表明，木耳双核体菌丝中某些酯酶同工酶基因位点在一定的发育时期才开始表达，老龄菌丝酯酶同工酶酶谱在木耳菌株鉴别和遗传育种研究中具有更大的应用价值。

ESTERASE ISOZYME ZYMOGRAM POLYMORPHISMS OFCULTIVATED STRAINS IN AURICULARIA AURICULA

Esterase isozyme zymogram polymorphisms of 10 cultivated strains in Auricularia auricula (Hook)Underw were inveshgated using vertical slab polyacrylthede gel electrophoresis. There were 25 bands of esteraseisozyme in the zymograms of young mycelia (7d) of 10 tested strains, each of 10 strains had 2 or 3 bandsrespechvely. The 10 strains were divided only into two groups according to their zymogram phenotypes. Theesterase isozyme zymograxns of old mycelia (72d) were signjficantly different from those of young mycelia,44bands existed in their zymograms,and each strainn had 3~6 bands respechvely. The 10 cultivated strains weredivided into eight groups according to their zymogram phenotypes. This result revealed that some esteraseisozyme genes of dikaryon begin to express in certain developmental stages. The esterase isozyme zymograzn ofold mycelia is more adaphve than that of young mycelia tO be used in the strain idenhfication. genetic distanceanalysis and genehc breeding of A. auricula.