| 三种鳞翅目幼虫肠道蛋白水解酶的活性 |
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| 引用本文： | 白成 沙槎云. 三种鳞翅目幼虫肠道蛋白水解酶的活性[J]. 昆虫学报, 1990, 33(3): 296-300 |
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| 作者姓名： | 白成 沙槎云 |
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| 作者单位： | 中国科学院动物研究所, 北京 |
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| 摘 要： | 本工作以酪蛋白为底物,测定粘虫Mythimna separata Walker、棉铃虫Heliothis armigera Hubner和大蜡螟 Galleria mellonlla Linnaeus三种鳞翅目幼虫肠道蛋白水解酶的活性,并分别用BTEE和TAME为底物,测定了其中类胰凝乳蛋白酶和类胰蛋白酶的活性.结果表明:三种幼虫肠道都含有类胰凝乳蛋白酶和类胰蛋白酶.抑制剂TPCK可以部分地抑制类胰凝乳蛋白酶的活性,而胰酶抑制剂则显著地抑制类胰蛋白酶的活性.
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| 关 键 词： | 粘虫 棉铃虫 大蜡螟 蛋白水解 类胰疑乳蛋白酶 类胰蛋白酶 |
ACTIVITIES OF DIGESTIVE PROTEASES OF THREE SPECIES OF LEPIDOPTEROUS LARVAE |
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| BAI CHENG SHA CHA-YUNInstustue of Zoology,Acadcmia Sinica. ACTIVITIES OF DIGESTIVE PROTEASES OF THREE SPECIES OF LEPIDOPTEROUS LARVAE[J]. Acta Entomologica Sinica, 1990, 33(3): 296-300 |
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| Authors: | BAI CHENG SHA CHA-YUNInstustue of Zoology Acadcmia Sinica |
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| Affiliation: | Institute of Zoology, Academia Sinica, Beijing |
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| Abstract: | The activities of digestive proteases of larvae of Mythimna separata, Heliothis armigera and Galleria mellonella were determined by using casein as the substrate The activities of chymotrypsin-like and trypsin-like enzymes were also determined with their specific substrates. The results showed that all the preparations from the larval intestines contained chymotrypsin-like and trypsin-like enzymes. The inhibitory actions of TPCK on the chymotrypsin-like enzyme and trypsin-inhibitor on the trypsin-like enzyme were also studied |
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| Keywords: | Myrhtmria teparata--Helioihis armigera--Gallena mellonella--protease--chymotrypsin--trypsin |
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