Phospholipid activation of Lactobacillus plantarum undecaprenyl pyrophosphate synthetase

The ability of a number of phospholipids to stimulate $\text{Lactobacillus}$$\text{plantarum}$ undecaprenyl pyrophosphate synthetase was investigated. The detergent Triton X-100, which is added to stabilize the enzyme during purification and is required for $\text{in}$$\text{vitro}$ activity, was removed with the non-ionic resin XAD-2. The effects of cardiolipin, phosphatidyl ethanolamine, phosphatidyl choline, and phosphatidyl glycerol on the activity of XAD-2 treated undecaprenyl pyrophosphate synthetase were determined. Of the phospholipids studied only cardiolipin stimulated $\text{in}$$\text{vitro}$ enzymic activity as effectively as Triton X-100.