Measurement of plasma histamine by stable isotope dilution gas chromatography-mass spectrometry: methodology and normal values |
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| Authors: | J J Keyzer B G Wolthers F A Muskiet H Breukelman H F Kauffman K de Vries |
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| Affiliation: | 1. Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands;2. the Department of Allergology, University Hospital, Groningen, The Netherlands |
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| Abstract: | A new method for the determination of histamine by stable isotope dilution mass fragmentography is described. The method is specific, sensitive, and accurate, resulting in a within-day coefficient of variation of 4.1% and a day-to-day variation of 7.9%. It was shown that the first blood sample after a venipuncture can contain an artificially elevated plasma histamine concentration. Platelets contain about 7 pmol histamine/10(9) cells. Serum histamine was elevated about four times in comparison with plasma histamine. This phenomenon was mainly ascribed to degranulation of basophilic leukocytes by complement activation during blood clotting. Normal values for plasma histamine were (n = 25) 2.07 +/- 0.75 nmol/liter (mean +/- 1 SD), which is one of the lowest values reported up to now. |
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| Keywords: | histamine gas chromatography-mass spectrometry plasma histamine serum histamine normal values mastocytosis |
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