Highly sensitive quantitative analysis of nicotianamine using LC/ESI-TOF-MS with an internal standard |
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| Authors: | Wada Yasuaki Yamaguchi Isomaro Takahashi Michiko Nakanishi Hiromi Mori Satoshi Nishizawa Naoko K |
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| Institution: | Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. |
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| Abstract: | A highly sensitive quantitative method for analyzing nicotianamine (NA) by liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) is reported. Fluorenylmethoxycarbonylation of nicotianamine reduced its polarity and enabled its retention in a reversed-phase column. The adoption of N(epsilon)-nicotyllysine (NL) as an internal standard ensured reliable quantification by giving a linear calibration curve drawn between the NA/NL molar ratios of standard solutions injected and the NA/NL area ratios in mass chromatograms. The high sensitivity of this analytical method allowed us to measure the amount of NA. This analytical method has applications to all research concerning NA. |
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