Gene expression and intracellular localization of somatolactin in the pituitary of rainbow trout |
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Authors: | Toyoji Kaneko Sho Kakizawa Takashi Yada Tetsuya Hirano |
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Affiliation: | (1) Ocean Research Institute, University of Tokyo, 1-15-1, Minamidai, Nakano, 164 Tokyo, Japan |
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Abstract: | The gene expression and intracellular localization of somatolactin (SL), a putative pituitary hormone structurally related to both growth hormone and prolactin, were investigated in the pituitary of rainbow trout, Oncorhynchus mykiss. Using an in situ hybridization technique, we demonstrated the gene expression of the SL molecule in cells bordering the neurohypophysial tissue in the pars intermedia. These cells were identified immunocytochemically as SL-cells on the adjacent section. Electron-microscopic immunocytochemistry by means of the protein A-gold technique, also revealed that the SL-immunoreactivity was located mostly on the secretory granules in SL-cells. Our findings clearly indicate that SL is biosynthesized and stored in the granules in these cells. |
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Keywords: | Somatolactin Pituitary Gene expression In situ hybridization Immunocytochemistry Protein A-gold Oncorhynchus mykiss (Teleostei) |
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