The virus-specific RNA species in free and membrane-bound polyribosomes of transformed cells replicating murine sarcoma-leukemia viruses |
| |
Authors: | G Shanmugam S Bhaduri M Green |
| |
Institution: | Institute for Molecular Virology St. Louis University School of Medicine St. Louis, Missouri 63110 USA |
| |
Abstract: | Ribonucleic acids extracted from polyribosomes of cells replicating murine sarcoma-leukemia viruses (M-MSV(MLV)) were resolved by electrophoresis on 2.5% polyacrylamide gels. Virus-specific RNA was detected by hybridization of RNA in the gel fractions with the 3H-DNA product of the viral RNA-directed DNA polymerase. The postmicrosomal supernatant and the free polyribosomes contained one peak of virus-specific RNA with a molecular weight of about 2.9 × 106 (35S). In contrast, the microsomes and the membrane-bound polyribosomes contained two peaks of virus-specific RNA in approximately equal amounts with molecular weights of 2.9 × 106 (35S) and 1.5 × 106 (approximately 20S). The high molecular weight viral RNA species might serve as polycistronic mRNA for the synthesis of large polypeptides that are cleaved to form the smaller viral proteins. |
| |
Keywords: | M-MSV(MLV) Moloney strain of murine sarcoma-leukemia virus PBS |
本文献已被 ScienceDirect 等数据库收录! |