| 母体外周血中胎儿有核红细胞的分离和富集方法的研究进展 |
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| 引用本文： | 梁卉,陈国杰,于燕,熊礼宽. 母体外周血中胎儿有核红细胞的分离和富集方法的研究进展[J]. 中华细胞与干细胞杂志(电子版), 2018, 8(1): 53-58. DOI: 10.3877/cma.j.issn.2095-1221.2018.01.010 |
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| 作者姓名： | 梁卉 陈国杰 于燕 熊礼宽 |
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| 作者单位： | 1. 518100，暨南大学附属深圳市宝安区妇幼保健院中心实验室；518100，深圳市出生缺陷研究重点实验室2. 450052，郑州大学附属第一医院消化内科3. 518100，暨南大学附属深圳市宝安区妇幼保健院中心产科 |
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| 基金项目： | 深圳市三名工程(SZSM201406007); 深圳市出生缺陷研究重点实验室(ZDSYS201504301707152); 深圳市知识创新计划基础研究项目(JCYJ20130402151000856); 深圳市宝安区医疗卫生基础研究项目(2013063，2014067，2017JD001) |
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| 摘 要： | 母体外周血中分离的胎儿有核红细胞（fNRBCs）包含胎儿完整的遗传信息，可用于无创产前诊断。fNRBCs的分离和富集方法主要分为三类：物理分选法、抗原-抗体结合分离法和增殖法。不同的方法获得的fNRBCs的数量和纯度不同，多种方法联合使用可以提高富集产物中fNRBCs的纯度和数量。本文就母体外周血中fNRBCs的分离和富集方法进行综述。
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| 关 键 词： | 胎儿 有核红细胞 外周血 无创产前诊断 分离 富集 |
| 收稿时间： | 2018-01-09 |
Progress in isolation and enrichment of fetal nucleated read blood cells from maternal peripheral blood |
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| Hui Liang,Guojie Chen,Yan Yu,Likuan Xiong. Progress in isolation and enrichment of fetal nucleated read blood cells from maternal peripheral blood[J]. , 2018, 8(1): 53-58. DOI: 10.3877/cma.j.issn.2095-1221.2018.01.010 |
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| Authors: | Hui Liang Guojie Chen Yan Yu Likuan Xiong |
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| Affiliation: | 1. Central Laboratory, Bao'an Maternal and Child Health Hospital, Jinan University, Shenzhen 518100, China; Shenzhen Key Laboratory of Birth Defects, Shenzhen 518100, China2. Department of Gastroenterology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China3. Department of Obstetrics, Bao'an Maternal and Child Health Hospital, Jinan University, Shenzhen 518100, China |
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| Abstract: | Fetal nucleated red blood cells (fNRBCs) from maternal peripheral blood have the whole genetic information of fetal, which could be used for non-invasive prenatal diagnosis. There are three sorts of methods for isolation and enrichment of fNRBCs: physical separation methods, antigen-antibody-dependent methods, and proliferation methods. The yield and purity vary from different methods. Combination of several methods could improve yield and purity of fNRBCs. In this review, we describe the progress in isolation and enrichment of fNRBCs from maternal peripheral blood. |
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| Keywords: | Fetal Nucleated red blood cells Peripheral blood Non-invasive prenatal diagnosis Isolation Enrichment |
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