Intron length variation of the Adh gene in Brachyscome (Asteraceae) |
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Authors: | Denda Tetsuo Kosuge Keiko Watanabe Kuniaki Ito Motomi Suzuki Yohei Short Philip S Yahara Tetsukazu |
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Institution: | (1) Department of Biology, Faculty of Science, Kobe University, 657 Kobe, Japan;(2) Department of Biology, Faculty of Science, Chiba University, 263 Chiba, Japan;(3) National Herbarium of Victoria, Birdwood Avenue, 3141 South Yarra, Victoria, Australia;(4) Department of Biology, Faculty of Science, Kyushu University, 812 Fukuoka, Japan |
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Abstract: | Eighteen polymerase chain reaction (PCR) products of the partial sequence of the Adh (alcohol dehydrogenase) gene from 10 Brachyscome species were sequenced and compared. These products contained the 5 three fourths of exon 4 and whole sequences of intron 3. They varied extensively in length due to the differences in length of intron 3. A total of 10 long insertions were flanked by direct repeats of 5 to 12 bp sequences, indicating inserted elements. These inserted elements were classified into the following five categories based on nucleotide sequence characteristics and length; (1) a region homologous to that of 5S RNA genes (5S DNA), (2) A-rich structure at the 3 end-like short interspersed elements (SINEs) in animals, (3) a sequence of 280 bp with no characteristic features, (4) a sequence of 125 bp with no characteristic features, (5) termini of 11 bp inverted repeats flanked by 5 bp sequence of direct repeats characteristics of a transposon. |
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Keywords: | Adh gene Brachyscome inserted elements plant SINEs transposons |
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