Energy-requiring uptake of protoberberine alkaloids by cultured cells ofThalictrum flavum |
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Authors: | H. Yamamoto M. Suzuki T. Kitamura H. Fukui M. Tabata |
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Affiliation: | (1) Faculty of Pharmaceutical Sciences, Kyoto University, 606 Kyoto, Japan;(2) Present address: Gifu Pharmaceutical University, Mitahora-higashi 5-6-1, 502 Gifu, Japan;(3) Present address: Bioscience Research Center, Mitsui Petrochemical Industries Ltd., Waki-cho, Kuga-gun, 740 Yamaguchi, Japan |
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Abstract: | Cultured cells ofThalictrum flavum take up berberine exogenously added to medium against the concentration gradient. This uptake was temperature-dependent and sensitive to plasma membrane-bound ATPase inhibitors such as sodium orthovanadate and diethylstilbestrol, indicating that the process is mediated by an energy-requiring system. The time-course of pH-shift during berberine uptake suggests the participation of a berberine-proton antiport system in the berberine uptake by the cultured cells. In addition, the existence of a specific transport system was suggested by the competitive inhibition of berberine uptake by berberine analogues, coptisine and jatrorrhizine. |
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