| 古尼拟青霉麦角甾醇高产液体培养条件及提取工艺的优化 |
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| 引用本文： | 蒋岚,于士军,张莹,张琛,樊美珍,李春如. 古尼拟青霉麦角甾醇高产液体培养条件及提取工艺的优化[J]. 菌物学报, 2012, 31(3): 413-421 |
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| 作者姓名： | 蒋岚 于士军 张莹 张琛 樊美珍 李春如 |
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| 作者单位： | 安徽农业大学安徽省微生物防治重点实验室 安徽合肥230036 |
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| 摘 要： | 以古尼拟青霉生物量和麦角甾醇含量为指标，对古尼拟青霉的高产麦角甾醇培养条件及麦角甾醇提取工艺进行了优化。正交试验结果表明，高产麦角甾醇的最适液体培养基为蔗糖4%，麸皮2%，KH2PO4 0.025%和NaCl 0.05%；响应面实验结果表明，麦角甾醇最佳提取工艺为：提取溶剂浓度为80%乙醇，液料比为200:1，提取时间为65min。
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| 关 键 词： | 古尼拟青霉 昆虫病原真菌 麦角甾醇 最适液体培养基 最适培养条件 |
Optimization of liquid culture conditions and extraction process of ergosterols from Paecilomyces gunnii |
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| JIANG Lan,YU Shi-Jun,ZHANG Ying,ZHANG Chen,FAN Mei-Zhen and LI Chun-Ru. Optimization of liquid culture conditions and extraction process of ergosterols from Paecilomyces gunnii[J]. Mycosystema, 2012, 31(3): 413-421 |
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| Authors: | JIANG Lan YU Shi-Jun ZHANG Ying ZHANG Chen FAN Mei-Zhen LI Chun-Ru |
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| Affiliation: | Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei, Anhui 230036, China;Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei, Anhui 230036, China;Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei, Anhui 230036, China;Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei, Anhui 230036, China;Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei, Anhui 230036, China;Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei, Anhui 230036, China |
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| Abstract: | Liquid culture conditions and extraction process of ergosterols from the entomopathogenic fungus Paecilomyces gunnii were optimized by using the indices of biomass and ergosterol productions. The result of orthogonal test indicated that the optimal liquid medium contained sucrose 4%, bran 2%, KH2PO4 0.025% and NaCl 0.05%. Response surface experiment demonstrated that the optimal extract solvent concentration was 80% ethanol, the ratio of solvent to raw material was 200:1 and the extract duration was 65min. |
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| Keywords: | Paecilomyces gunnii entomopathogenic fungus ergosterols optimal liquid medium optimal culture conditions |
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