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| 蛹虫草水溶性多糖含量测定方法的比较与优化 | |
| 引用本文： | 薛俊杰,唐庆九,刘艳芳,周帅,赵明文,张劲松. 蛹虫草水溶性多糖含量测定方法的比较与优化[J]. 菌物学报, 2012, 31(3): 443-449 |
| 作者姓名： | 薛俊杰 唐庆九 刘艳芳 周帅 赵明文 张劲松 |
| 作者单位： | 1. 国家食用菌工程技术研究中心农业部应用真菌资源与利用重点开放实验室上海市农业遗传育种重点开放实验室上海市农业科学院食用菌研究所 上海201403;南京农业大学生命科学学院农业部农业微生物环境工程重点开放实验室 江苏南京210095 2. 国家食用菌工程技术研究中心农业部应用真菌资源与利用重点开放实验室上海市农业遗传育种重点开放实验室上海市农业科学院食用菌研究所 上海201403 3. 南京农业大学生命科学学院农业部农业微生物环境工程重点开放实验室 江苏南京210095 |
| 基金项目： | 上海市科技兴农重点攻关项目”香菇、灰树花、北冬虫夏草种质创新及其生产加工关键技术研究”[沪农科攻字（2009）第2-2号] |
| 摘 要： | 已报道文献中蛹虫草多糖含量差异非常大，通过比较分析发现差异主要在于干扰物质的去除。结合高效阴离子色谱对干扰物质的测定，对常见的几种蛹虫草多糖提取测定方法进行了分析比较，在此基础上建立了去除干扰物的蛹虫草多糖测定方法。该方法简便、快速、精确，经系统的方法学考察证明，是一种分析蛹虫草多糖较好的方法。 |
| 关 键 词： | 蛹虫草 多糖 苯酚硫酸法 |
Comparison and optimization of the methods for analyzing the water soluble polysaccharides in Cordyceps militaris |
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| XUE Jun-Jie,TANG Qing-Jiu,LIU Yan-Fang,ZHOU Shuai,ZHAO Ming-Wen and ZHANG Jing-Song. Comparison and optimization of the methods for analyzing the water soluble polysaccharides in Cordyceps militaris[J]. Mycosystema, 2012, 31(3): 443-449 | |
| Authors: | XUE Jun-Jie TANG Qing-Jiu LIU Yan-Fang ZHOU Shuai ZHAO Ming-Wen ZHANG Jing-Song |
| Affiliation: | National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of A;National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of A;National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of A;National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of A;Key Laboratory of Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of A |
| Abstract: | There are significant differences of the polysaccharide content from Cordyceps militaris assayed with different methods in previous literatures, which could be mainly caused by removing the interfering substances or not. After monitoring the interfering substances by high performance anion exchange chromatography, two common analysis methods for determination of the polysaccharides of C. militaris were compared. We found that the extraction with 80% ethanol in twice could effectively remove the interfering substance. Our study showed that it was a sensitive, accurate and reproducible method and could be successfully applied to analyze the water soluble polysaccharides produced by C. militaris. |
| Keywords: | Cordyceps militaris polysaccharide phenol-sulfuric acid procedure |
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