小鼠造血干细胞增殖与分化性能的研究

本文选择Ｙ染色体特异的性别决定基因作为新的细胞遗传标志，采用ＰＣＲ技术研究了小鼠造血干细胞的增殖与分化性能。将雄鼠骨髓细胞输注给经致死剂量射线照射的雌性受体小鼠、ＰＣＲ测试结果表明，所有ＣＦＵ－Ｓ均为供体起源。供体来源的ＣＦＵ－Ｓ在其输入体内后，可通过增殖，分化形成各系造血细胞，但ＣＦＵ－Ｓ中的纤维母细胞和ＣＦＵ－Ｓ重建造血后受体小鼠骨髓中的纤维母细胞均为受体起源。由此可见，小鼠骨髓中的ＣＦＣ－Ｓ

STUDIES ON CHARACTERISTICS OF PROLIFERATION AND DIFFERENTIATION OF HEMATOPOIETIC STEM CELLS IN MICE

By using Y chromosome specific sex-determining region (Sry) as a new cytogenetic marker and PCR technique,the characteristics of proliferation and differentiation ofhematopoietic stem cells in mice were studied. Bone marrow cells from male mice wereinjected into lethally-irradiated female mice,PCR results indicated that all of the CFU-S were originated from donor. It was found that CFU-S was able to proliferate and differentiate into various hematopoietic cells in vivo during its transplantation. Whereas thefibroblasts within donor CFU-S and the fibroblasts from bone marrow in recipientmouse reestablished by donor CFU-S were shown to be originated from the recipient.The above data demonstrated that CFC-S from bone marrow in mice possessed the characteristics of hematopoietic stem cells but was not prone to differentiate into fibroblasts.