Anaerobic utilization of pectinous substrates at extremely haloalkaline conditions by Natranaerovirga pectinivora gen. nov., sp. nov., and Natranaerovirga hydrolytica sp. nov., isolated from hypersaline soda lakes |
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Authors: | D Y Sorokin T P Tourova A N Panteleeva E N Kaparullina G Muyzer |
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Institution: | (1) Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia;(2) Environmental Biotechnology Group, Department of Biotechnology, Delft University of Technology, Delft, The Netherlands;(3) Bioengineering Centre, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/1, 117811 Moscow, Russia;(4) Institute of Physiology and Biochemistry of Microorganisms, Russian Academy of Sciences, Puschino, Russia;(5) Present address: Department of Aquatic Microbiology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands |
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Abstract: | Anaerobic enrichments at pH 10, with pectin and polygalacturonates as substrates and inoculated with samples of sediments
of hypersaline soda lakes from the Kulunda Steppe (Altai, Russia) demonstrated the potential for microbial pectin degradation
up to soda-saturating conditions. The enrichments resulted in the isolation of six strains of obligately anaerobic fermentative
bacteria, which represented a novel deep lineage within the order Clostridiales loosely associated with the family Lachnospiraceae. The isolates were rod-shaped and formed terminal round endospores. One of the striking features of the novel group is a
very narrow substrate spectrum for growth, restricted to galacturonic acid and its polymers (e.g. pectin). Acetate and formate
were the final fermentation products. Growth was possible in a pH range from 8 to 10.5, with an optimum at pH 9.5–10, and
in a salinity range from 0.2 to 3.5 M Na+. On the basis of unique phenotypic properties and distinct phylogeny, the pectinolytic isolates are proposed to be assigned
to a new genus Natranaerovirga with two species N. hydrolytica (APP2T=DSM24176T=UNIQEM U806T) and N. pectinivora (AP3T=DSM24629T=UNIQEM U805T). |
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