Genetic analysis and molecular mapping of maize (Zea mays L.) stalk rot resistant gene Rfg1 |
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| Authors: | D.?E.?Yang,C.?L.?Zhang,D.?S.?Zhang,D.?M.?Jin,M.?L.?Weng,S.J.?Chen,H.?Nguyen,B.?Wang author-information" > author-information__contact u-icon-before" > mailto:bwang@genetics.ac.cn" title=" bwang@genetics.ac.cn" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
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| Affiliation: | (1) Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101 Beijing, China;(2) China Agricultural University, 100094 Beijing, China;(3) Texas Tech University, Lubbock, TX 79401, USA |
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| Abstract: | One single pathogen Fusarium graminearum Schw. was inoculated to maize inbred lines 1,145 (Resistant) and Y331 (Susceptive), and their progenies of F1, F2 and BC1F1 populations. Field statistical data revealed that all of the F1 individuals were resistant to the disease and that the ratio of resistant plants to susceptive plants was 3:1 in the F2 population, and 1:1 in the BC1F1 population. The results revealed that a single dominant gene controls the resistance to F. graminearum Schw.. The resistant gene to F. graminearum Schw. was denominated as Rfg1 according to the standard principle of the nomenclature of the plant disease resistant genes. RAPD (randomly amplified polymorphic DNA) combined with BSA (bulked segregant analysis) analysis was carried out in the developed F2 and BC1F1 populations, respectively. Three RAPD products screened from the RAPD analysis with 820 Operon 10-mer primers showed the linkage relation with the resistant gene Rfg1. The three RAPD amplification products (OPD-201000, OPA-041100 and OPY-04900) were cloned and their copy numbers were determined. The results indicated that only OPY-04900 was a single-copy sequence. Then, OPY-04900 was used as a probe to map the Rfg1 gene with a RIL F7 mapping population provided by Henry Nguyen, which was developed from the cross  S3×Mo17 . Rfg1 was primarily mapped on chromosome 6 between the two linked markers OPY-04900 and umc21 (Bin 6.04–6.05). In order to confirm the primary mapping result, 25 SSR (simple sequence repeat) markers and six RFLP (restriction fragment length polymorphism) markers in the Rfg1 gene-encompassing region were selected, and their linkage relation with Rfg1 was analyzed in our F2 population. Results indicated that SSR marker mmc0241 and RFLP marker bnl3.03 are flanking the Rfg1 gene with a genetic distance of 3.0 cM and 2.0 cM, respectively. This is the first time to name and to map a single resistant gene of maize stalk rot through a single pathogen inoculation and molecular marker analysis.Communicated by H.F. Linskens |
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