麻醉剂MS-222对斑马鱼行为的影响

实验室条件下观察并测定了斑马鱼(Danio rerio)在不同MS-222浓度处理下的麻醉行为、斑马鱼在高剂量MS-222致死过程中的行为变化、MS-222对斑马鱼摄食条件反射的影响。试验表明:(1)斑马鱼的麻醉行为是一个渐变的过程,可分为不被麻醉(Ⅰ)、轻度麻醉(Ⅱ)、中度麻醉(Ⅲ)深度麻醉(Ⅳ);(2)不同MS-222浓度下斑马鱼进入不同麻醉程度的时间有差异;(3)经MS-222处理900s过程中,80-90mg·L^-1浓度组进入Ⅲ级麻醉程度并有100%的存活率,而麻醉浓度在100mg·L^-1以上时可以迅速在49s内使鱼进入Ⅲ级麻醉程度以及在178s后即可进入Ⅳ级麻醉程度,并在中度麻醉和深度麻醉的过渡中死亡,存活率不超过10%;(4)MS-222对摄食条件反射影响的试验中,光刺激-不麻醉、无光刺激-不麻醉、光刺激-麻醉、无光刺激-麻醉四组班马鱼从投喂到摄食所用时间分别为12.06±1.34s、13.20±1.13s、56.56±56.48s、36.20±25.74s,麻醉组的摄食速度慢于对照组,说明MS-222影响了斑马鱼的摄食条件反射。

The effects of MS-222 on behavior of Danio rerio

The effects of MS-222, an anaesthetic for fish, on behaviors of Danio rerio were examined under laboratory condition.The results showed that the behavior of anaesthotized D.rerio was a gradual process, and four narcotic stages of fish behavior, non-anarcosis(Ⅰ), light anarcosis(Ⅱ), mediate anarcosis(Ⅲ), and deep anarcosis(Ⅳ), were defined in the present study.The time of the fish being in a certain narcotic stage was not familiar under different concentrations of MS-222.When exposing to high dosage of anaesthetic for 15min, fish in the water with MS-222 concentration of 80-90 mg·L^-1 was in Ⅲ narcotic stage and all fish survived, but more than 90%of fish above 100 mg·L^-1 of MS-222 finally died.In the experiment of conditional reflex of feeding, four treatments, photic stimulation but not anesthesia (P), neither photic stimulation nor anesthesia (NN), photic stimulation and anesthesia (PA), and not photic stimulation but anesthesia (A), were set.The time from putting the feed into the water to fish eating the feed in treatment P, NN, PA, and A was 12.06±1.34s, 13.20±1.13s, 56.56±56.48s and 36.20±25.74s, respectively.It was shown that MS-222 prolonged the time of conditional reflex.The present research could give an advice in using MS-222 in experiments and fish transportation.