Application of carbon nanotubes for detecting anti-hemagglutinins based on antigen-antibody interaction |
| |
Authors: | Takeda Seiji Sbagyo Agus Sakoda Yoshihiro Ishii Atsushi Sawamura Makoto Sueoka Kazuhisa Kida Hiroshi Mukasa Koichi Matsumoto Kazuhiko |
| |
Institution: | Graduate School of Engineering, Hokkaido University, Kita 13 Nishi 8 Kita-ku, Sapporo 060-8628, Japan. takeda@nano.eng.hokudai.ac.jp |
| |
Abstract: | Carbon nanotube sensors detected anti-hemagglutinin binding to immobilized hemagglutinins. An ultra-sensitive detection method for antibodies or antigens in serum is required. Hemagglutinins were immobilized on the reverse side of a carbon nanotube, thereby producing a source and a drain. Electrode pads covered each edge of the nanotube. The I-V curves between the source and the drain were measured after incubation of anti-hemagglutinins with immobilized hemagglutinins in a buffered solution on the reverse side of the nanotube. The sensitivity of the CNT sensor was higher than that of an ELISA system. This method constitutes a new tool to analyze interaction among biomolecules on a substrate. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|