20-Hydroxyeicosatetraenoic acid is excreted as a glucuronide conjugate in human urine. |
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Authors: | C Prakash J Y Zhang J R Falck K Chauhan I A Blair |
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Affiliation: | Department of Pharmacology, Vanderbilt University, Nashville, TN 37232. |
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Abstract: | 20-hydroxyeicosatetraenoic acid, a major renal P-450 metabolite of arachidonic acid, has been quantified in human urine using capillary gas chromatography/electron capture negative ion chemical ionization mass spectrometry. The urinary excretion of 20-hydroxyeicosatetraenoic acid was in the low pg/ml range. However, treatment of urine with beta-glucuronidase resulted in a 13- to 28-fold increase in its concentration. This suggests 20-hydroxyeicosatetraenoic acid differs from other eicosanoids in that it is excreted primarily as a glucuronide conjugate. |
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