Autophagy inhibition in early but not in later stages prevents 3T3-L1 differentiation: Effect on mitochondrial remodeling |
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| Authors: | Vojtech Skop Monika Cahova Helena Dankova Zuzana Papackova Eliska Palenickova Petr Svoboda Jarmila Zidkova Ludmila Kazdova |
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| Affiliation: | 1. Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic;2. Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Prague, Czech Republic;3. Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic |
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| Abstract: | Autophagy is essential for successful white adipocyte differentiation but the data regarding the timing and relevance of autophagy action during different phases of adipogenesis are limited. |
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| Keywords: | 3-MA, 3-methyladenine Acadl, acyl-coenzyme A dehydrogenase, long-chain Asn, l-asparagine Atp5b, ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit BAT, brown adipose tissue CPT1b, carnitine palmitoyltransferase 1b CS, citrate synthase Dex, dexamethasone DM, differentiation medium DMEM, Dulbecco modified Eagle medium EDTA, ethylenediaminetetraacetic acid FABP4, fatty acid binding protein 4 FM, fibroblast medium GOI, gene of interest Hspa9, heat shock protein 9 IBMX, 1-methyl-3-isobutylxanthine Ins, insulin LC3, microtubule-associated protein 1A/1B-light chain 3 Mdh2, malate dehydrogenase 2, NAD (mitochondrial) MEFs, mouse embryonic fibroblasts Mt, mitochondria Ndufs1, NADH dehydrogenase (ubiquinone) Fe-S protein 1 Pcx, pyruvate carboxylase PMSF, phenylmethanesulfonyl fluoride PPARγ, peroxisome proliferator-activated receptor gamma PVDF, polyvinylidene difluoride Sdhb, succinate dehydrogenase complex, subunit B, iron sulfur (Ip) SDS, sodium dodecyl sulfate Slc25a1, solute carrier family 25 (mitochondrial carrier, citrate transporter), member 1 TNB,, thionitrobenzoic acid Uqcrc1, ubiquinol-cytochrome c reductase core protein 1 WAT, white adipose tissue |
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