Role of a conserved membrane glycine residue in a dicarboxylate transporter from Sinorhizobium meliloti |
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| Authors: | Trainer Maria A Yurgel Svetlana N Kahn Michael L |
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| Affiliation: | Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA. |
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| Abstract: | Nitrogen-fixing rhizobial bacteroids import dicarboxylates by using the DctA transporter. G114 of DctA is highly conserved. A G114D mutant is inactive, but DctA with a small amino acid (G114A) or a helix disrupter (G114P) retains significant activity. G114 probably interacts with other membrane helices in stabilizing a substrate-binding pocket. |
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