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Effects of DTT and thioredoxins on rat argininosuccinate synthetase activity in vitro |
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| Authors: | J Demarquoy A P Balangé R Vaillant C Gautier |
| Abstract: | Argininosuccinate synthetase (ASS, EC 6.3.4.5), the third enzyme of urea-cycle, was studied in desactivated extracts of rat liver. The enzyme is activated, in vitro, by Mg2+ ions (5 mM) and dithiothreitol (DTT: 10 mM). After reduction by DTT, thioredoxins isolated from rat liver were able to activate ASS by 370%. |
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