Anisotropy measurements of intrinsic fluorescence of prenyllipids reveal much higher mobility of plastoquinol than alpha-tocopherol in model membranes |
| |
Authors: | Jemioła-Rzemińska Małgorzata Kruk Jerzy Strzałka Kazimierz |
| |
Affiliation: | Department of Plant Physiology and Biochemistry, The Jan Zurzycki Institute of Molecular Biology and Biotechnology, Jagiellonian University, Gronostajowa Street 7, 30-387, Krakow, Poland |
| |
Abstract: | As an alternative to a fluorescent probe approach, the intrinsic fluorescence of reduced forms of prenylquinones has been exploited, which offers a convenient means of determining directly motional properties of these molecules. The steady-state fluorescence anisotropy measurements of plastoquinols (PQH(2)) and alpha-tocopherol (alpha-Toc) incorporated into phospholipid liposomes have been performed. The effect of prenyllipid concentration, PQH(2) side chain length and the composition of the membranes has been studied. For the data interpretation, the fundamental anisotropy of alpha-Toc, PQH(2), ubiquinol-10 and alpha-tocopherolquinol, as well as the angles between the absorption and emission transition moments have been also determined. It was concluded that alpha-Toc shows very low mobility in the lipid bilayer, whereas PQH(2)-9 displays significant motional freedom in dipalmitoylphosphatidylcholine vesicles and even higher in egg yolk lecithin membranes. |
| |
Keywords: | Prenyllipids Model membranes Steady-state fluorescence anisotropy Intrinsic fluorescence |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|