Transformation of the yeast Saccharomyces kluyveri by Saccharomyces cerevisiae-based plasmids |
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Authors: | Hiroaki Fujimura |
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Affiliation: | Laboratory for Molecular Biology, Pharma Research Laboratories, Hoechst Japan Limited, Kawagoe. |
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Abstract: | For the transformation of the yeast Saccharomyces kluyveri, ura3 mutants were obtained by 5-fluoro-orotic acid selection. By utilizing the method based on treatment of intact cells with alkali cations, the ura3 strains of S. kluyveri were transformed by Saccharomyces cerevisiae-based plasmids. In the transformed cells, a S. cerevisiae centromere-based plasmid was stably replicated autonomously. Thus, this system will permit the study of gene expression and its regulation in S. kluyveri in relationship to that in S. cerevisiae. |
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Keywords: | Transformation Saccharomyces kluyveri Saccharomyces cerevisiae Plasmid Uracil auxotroph |
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