Eliminating primers from completed polymerase chain reactions with exonuclease VII. |
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Authors: | H H Li X F Cui N Arnheim |
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Affiliation: | University of Southern California, Los Angeles 90089-1340. |
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Abstract: | Single-stranded oligonucleotide primers can be efficiently removed after PCR using E.coli exonuclease VII. Even only a few molecules of double stranded PCR product are unaffected by a treatment which eliminates 20 picomoles of primer in the presence of 500 ng of denatured genomic DNA. Exonuclease VII treatment is rapid and could simplify complicated multistep PCR protocols. |
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