Development of a novel Sinapis arvensis disomic addition line in Brassica napus containing the restorer gene for Nsa CMS and improved resistance to Sclerotinia sclerotiorum and pod shattering |
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Authors: | Wenhui Wei Yunchang Li Lijun Wang Shengyi Liu Xiaohong Yan Desheng Mei Yinde Li Yusong Xu Pengfei Peng Qiong Hu |
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Institution: | (1) Key Laboratory for Biological Sciences of Oil Crops, National Center for Oil Crops Improvement, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, 430062 Wuhan, People’s Republic of China |
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Abstract: | An allo-cytoplasmic male sterile line, which was developed through somatic hybridization between Brassica napus and Sinapis arvensis (thus designated as Nsa CMS line), possesses high potential for hybrid production of rapeseed. In order to select for restorer lines, fertile plants
derived from the same somatic hybridization combination were self-pollinated and testcrossed with the parental Nsa CMS line for six generations. A novel disomic alien addition line, B. napus–S. arvensis, has been successfully developed. GISH analysis showed that it contains one pair of chromosomes from S. arvensis and 19 pairs from B. napus, and retains stable and regular mitotic and meiotic processes. The addition line displays very strong restoration ability
to Nsa CMS line, high resistance to Sclerotinia sclerotiorum and a low incidence of pod shattering. Because the addition line shares these very important agricultural characters, it
is a valuable restorer to Nsa CMS line, and is named NR1 here (Nsa restorer no. 1). |
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