Immobilization of pepsinogen on Sepharose |
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| Authors: | K. M. Shamsuzzaman N. F. Haard |
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| Affiliation: | (1) Department of Biochemistry, Memorial university of Newfoundland, A1B 3X9 St. John's, Newfoundland, Canada |
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| Abstract: | Summary Porcine pepsinogen was attached to CNBr-activated-Sepharose 4B and self-activated to form Sepharose-pepsin. The maximum loading of pepsin on Sepharose was about 43 mg of pepsin per g of freeze-dried product. Sepharose-pepsin was stable on exposure to protein substrates and had a specific activity similar to that of the free enzyme. |
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