New primers for amplifying and sequencing the mitochondrial ND4/ND5 gene region of the Cypriniformes (Actinopterygii: Ostariophysi) |
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| Authors: | Masaki Miya Kenji Saitoh Robert Wood Mutsumi Nishida Richard L Mayden |
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| Institution: | (1) Department of Zoology, Natural History Museum & Institute, Chiba, 955-2 Aoba-cho, Chuo-ku, Chiba 260-8682, Japan;(2) Tohoku National Fisheries Research Institute, 3-27-5 Shinhama, Shiogama, Miyagi 985-0001, Japan;(3) Department of Biology, St. Louis University, 357 Laclede Ave., St. Louis, MO 63103-2010, USA;(4) Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164-8639, Japan |
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| Abstract: | We provide 15 new primers for amplifying and sequencing the mitochondrial ND4/ND5 gene region of the Cypriniformes in an attempt
to resolve relationships of this diverse group of freshwater fishes with extensive taxonomic sampling. Sequences from this
region have the following desirable characteristics for phylogenetic analyses, some of which are lacking from the more commonly
used cyt b and 12S/16S rRNA genes: they are (1) easy to align, (2) relatively long (ca. 3.4 kb), and (3) contain more phylogenetically
informative variation at 1st and 2nd codon positions. Moreover, the ND4/ND5 gene region is easy to amplify and sequence when
employing the protocol suggested herein. |
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| Keywords: | Cypriniformes Tree of Life (CToL) Molecular systematics Phylogeny |
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