Glycosylation of the alpha and beta tubulin by sialyloligosaccharides |
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Authors: | Hino Mizuki Kijima-Suda Isao Nagai Yoshitaka Hosoya Hiroshi |
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Institution: | Department of Biological Science, Graduate School of Science, Hiroshima University, Hiroshima, Japan. |
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Abstract: | To examine whether alpha and beta tubulin are glycoproteins, we used a pyridylamino labeling method and a monoclonal antibody, SG3-1, raised against NeuAcalpha2-3Gal structure. Alpha and beta tubulin from both pig brain and HeLa cells were positive for the SG3-1 antibody by immunoblot assay. Sialidase treatment reduced the reactivity of the SG3-1 antibody to alpha and beta tubulin molecules. N-linked oligosaccharide analysis also showed that alpha and beta tubulin are glycosylated. Moreover, immunofluorescence analysis showed that the filamentous structure recognized by the SG3-1 antibody was overlapped with microtubules, especially in the vicinity of the nucleus. These results indicate that alpha and beta tubulin are glycosylated with sialyloligosaccharides. |
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