Hexavalent-chromium reduction by a chromate-resistantBacillus sp. strain |
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Authors: | Jesus Campos Mauro Martinez-Pacheco Carlos Cervantes |
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Institution: | (1) Instituto de Investigaciones Quimico-Biologicas, Universidad Michoacana, Edificio B-1, 58060 Morelia, Mich., Mexico |
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Abstract: | Bacillus strain QC1-2, isolated from a chromium-polluted zone, was selected by its high ability to both tolerate and reduce hexavalent chromium Cr(VI)] to less-toxic trivalent chromium Cr(III)]. Cell suspensions of strain QC1-2 rapidly reduced Cr(VI), in both aerobic and anaerobic conditions, to Cr(III) which remained in the supernatant. Cr(VI) reduction was dependent on the addition of glucose but sulfate, an inhibitor of chromate transport, had no effect. Studies with permeabilized cells and cell extracts showed that the Cr(VI) reductase of strain QC1-2 is a soluble NADH-dependent enzyme. |
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Keywords: | chromate reduction chromate resistance Bacillus |
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