DNA微阵列基片的化学处理

对于合成后点样的DNA微阵列 ,基片的表面化学处理非常重要。它直接影响到样品与基片的结合效率 ,进而影响杂交结果。基片表面的各种化学修饰方法多种多样 ,物理吸附主要以赖氨酸包被为主。共价结合通常使用同源偶联分子或异源偶联分子 ,还可以在基片表面组装线状、分支状连接分子或包被琼脂糖。着重介绍了DNA微阵列的制备 ,即样品如何固定到玻璃基片上。总结了不同类型基片表面的化学修饰方法以及DNA与基片的化学结合。

Chemical Dispose of Glass Slide in DNA Microarray

Chemical dispose of glass slide is vital to DNA microarray prepared by pre synthesized nucleic acid ,it directly affects the binding efficiency of DNA sample to glass slide.Polylysine coated glass slide attaches nucleic acid through physical absorption.Heterobifunctional or homobifunctional crosslinker are used in chemical covalent attachment.Different kinds of linear and branched ,dendrimeric linker with can be assembled.Agrose coated glass slide is also reported.The paper depict some practical methods of glass modification and how does the DNA sample immobilize onto glass slides.;