香烟烟雾提取物对小鼠气管环上皮钙粘附素表达的影响

体外进行器官培养，通过免疫组织化学和原位杂交方法检测香烟烟雾提取物（CSE）作用48、72h，小鼠气管上皮细胞上皮钙粘附素（E－cd）表达的变化。结果表明，正常对照组E－cd分布于气管假复层纤毛柱状上皮细胞连接处的胞膜上，50％CSE作用后，膜上E－cd表达减少，随着作用时间延长，胞浆内E-cd表达明显增加（P＜0.05），而细胞内E－cd mRNA含量无明显变化。提示，CSE对小鼠气管上皮细胞E-cd表达的调控在翻译后水平，胞膜上E－cd表达的下调与吸烟所致气道上皮细胞的损伤修复过程相关。

EFFECTS OF CIGARETTE SMOKE EXTRACT ON E CADHERIN EXPRESSION OF MICE TRACHEA

Mouse trachea was cultured in vitro, exposed to cigarette smoke extract (CSE), the change of E cadherin(E cd) expression on tracheal epithelial cells was determined by immunohistochemistry and in situ hybridization. The results showed that E cd was distributed on the membrane at the cell junctions of tracheal pseudostratified ciliated columnar epithelium. After exposure to 50% CSE, the membranous E cd expression decreased, the cytoplasmic E cd expression increased ( P <0 05) as the exposure time prolonged. But the content of E cd mRNA was not changed. It suggests that E cd expression is regulated at the posttranslational level, the downregulation of membranous E cd expression is related with injury and repair of tracheal epithelial cells caused by smoking.