Nisin biosynthesis genes are encoded by a novel conjugative transposon |
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Authors: | Nikki Horn Simon Swindell Helen Dodd Michael Gasson |
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Affiliation: | (1) Norwich Laboratory, AFRC Institute of Food Research, Colney Lane, NR4 7UA Norwich, UK |
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Abstract: | Summary Genes for biosynthesis of the lactococcal peptide antibiotic nisin were shown to be encoded by a novel chromosomally located transposon Tn5301. The element is 70 kb in size and lacks inverted repeats at its termini. Although a copy of the insertion sequence IS904 is located near to one end, this did not appear to be involved in the transposition process. The integrated element is flanked by the directly repeated sequence 5-TTTTTG-3. Analysis of ten independent transconjugants revealed that Tn5301 integration is site-specific; two chromosomal targets were identified and shown to have some sequence homology. The element shares features with the Tn916 family of conjugative transposons and with Tn554 but is also exhibits some unique properties. Tn5301 is thus considered to be the prototype of a novel class of conjugative transposon. |
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Keywords: | Nisin Transposon Conjugation Sucrose Lactococcus |
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