A silica sands-based method for faithful analysis of microbial communities and DNA isolation from a wide range of species |
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| Authors: | Xia Liu Yongdong Xu Zhi Li Shengwei Jiang Shuo Yao |
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| Institution: | 1. Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China;2. Qingshan Protection Bureau, Forestry Department of Liaoning Province, Shenyang, China |
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| Abstract: | A silica sands-based method has been developed to isolate high quality genomic DNAs from cells of animals, plants and microorganisms, such as Hemisalanx prognathus, Spinacia oleracea, Pichia pastoris, Bacillus licheniformis and Escherichia coli. To the best of our knowledge, no DNA isolation method has so wide application until now. In addition, this method and a commercially available kit were compared in analysis of microbial communities using high-throughput 16s rDNA sequencing. As a result, the silica sands-based method was found to be even more efficient in isolating genomic DNA from gram-positive bacteria than the kit, indicating that it would become a very valuable choice to faithfully reflect the composition of microbial communities. |
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| Keywords: | 16S rDNA sequencing environmental microorganisms genomic DNA extraction gram-positive bacteria microbial communities soil microorganisms |
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