Genetic mapping and QTL analysis of horticultural traits in cucumber (<Emphasis Type="Italic">Cucumis sativus</Emphasis> L.) using recombinant inbred lines |
| |
| Authors: | G?Fazio Email author" target="_blank">J?E?StaubEmail author M?R?Stevens |
| |
| Institution: | (1) USDA ARS/Dept. of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA,;(2) Department of Plant and Animal Sciences, 275 WIDB, Brigham Young University, Provo, UT 84602, USA,;(3) Former graduate student; currently at USDA ARS, Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, |
| |
| Abstract: | A set of 171 recombinant inbred lines (RIL) were developed from a narrow cross in cucumber (Cucumis sativus L.; 2n = 2x = 14) using the determinate (de), gynoecious (F), standard-sized leaf line G421 and the indeterminate, monoecious, little-leaf (ll) line H-19. A 131-point genetic map was constructed using these RILs and 216 F2 individuals to include 14 SSRs, 24 SCARs, 27 AFLPs, 62 RAPDs, 1 SNP, and three economically important morphological F (gynoecy), de (determinate habit), ll (little leaf)] markers. Seven linkage groups spanned 706 cM with a mean marker interval of 5.6 cM. The location of F and de was defined by genetic linkage and quantitative trait locus (QTL) analysis to be associated with SSR loci CSWCT28 and CSWCTT14 at 5.0 cM and 0.8 cM, respectively. RIL-based QTL analysis of the number of lateral branches in three environments revealed four location-independent factors that cumulatively explained 42% of the observed phenotypic variation. QTLs conditioning lateral branching (mlb1.1), fruit length/diameter ratio (ldr1.2) and sex expression (sex1.2) were associated with de. Sex expression was influenced by three genomic regions corresponding to F and de both on linkage Group 1, and a third locus (sex6.1) on linkage Group 6. QTLs conditioning the number of fruit per plant (fpl1.2), the number of lateral branches (mlb1.4) and fruit length/diameter ratio (ldr1.3) were associated with ll. The potential value of these marker-trait associations (i.e., yield components) for plant improvement is portended by the relatively high LOD scores (2.6 to 13.0) and associated R2 values (1.5% to 32.4%) that are affiliated with comparatively few genetic factors (perhaps 3 to 10).Communicated by H.C. BeckerMention of trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA and does not imply its approval to the exclusion of other products that may be suitable |
| |
| Keywords: | Linkage Analysis Yield Components Linkage Map Composite Interval Mapping Pleiotropic Effects |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
