Enzymatic depolymerization of N-succinylchitosan |
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Authors: | A. V. Il’ina V. P. Varlamov |
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Affiliation: | (1) Center of Bioengineering, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 1, Moscow, 117312, Russia |
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Abstract: | A complex of chitinolytic enzymes of Streptomyces kurssanovii and also lysozyme and Celloviridin, an industrial cellulase preparation, were demonstrated to provide for an enzymatic hydrolysis of N-succinylchitosan. Our studies were carried out on a high-molecular N-succinylchitosan with M of 390 kDa and a substitution degree of 0.8 as a substrate. All the enzymatic preparations were shown to be suitable for the obtaining of low-molecular derivatives of N-succinylchitosan. The complex of enzymes from S. kurssanovii showed the greatest activity: they reduced the characteristic viscosity of initial solution of the substrate by 78% for 30 min. A biodegradation of N-succinylchitosan of various molecular masses was shown to proceed under the action of lysozyme, and the cleavage reaction was revealed to decelerate at a decrease of the polymer molecular mass. A use of N-succinylchitosan in a complex with drugs for a prolongation of their action in a live organism was presumed. |
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Keywords: | chitin chitinases chitosan lysozyme N-succinylchitosan enzymatic depolymerization biodegradation |
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