Isolation and characterization of a fungus <Emphasis Type="Italic">Aspergillus</Emphasis> sp. strain F-3 capable of degrading alkali lignin |
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Authors: | Email author" target="_blank">Y?S?YangEmail author J?T?Zhou H?Lu Y?L?Yuan L?H?Zhao |
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Institution: | (1) School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, 116024, China;(2) School of Environmental and Chemical Engineering, Dalian University, Dalian, 116622, China |
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Abstract: | A fungus strain F-3 was selected from fungal strains isolated from forest soil in Dalian of China. It was identified as one
Aspergillus sp. stain F-3 with its morphologic, cultural characteristics and high homology to the genus of rDNA sequence. The budges or thickened node-like structures are peculiar structures of hyphae of the strain. The fungus degraded 65% of
alkali lignin (2,000 mg l−1) after day 8 of incubation at 30°C at pH 7. The removal of colority was up to 100% at 8 days. The biodegradation of lignin
by Aspergillus sp. F-3 favored initial pH 7.0. Excess acid or alkali conditions were not propitious to lignin decomposing. Addition of ammonium
l-tartrate or glucose delayed or repressed biodegradation activities. During lignin degradation, manganese peroxidase (28.2
U l−1) and laccase (3.5 U l−1)activities were detected after day 7 of incubation. GC-MS analysis of biodegraded products showed strain F-3 could convert
alkali lignin into small molecules or other utilizable products. Strain F-3 may co-culture with white rot fungus and decompose
alkali lignin effectively. |
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