Comparative analysis of the informativeness of ISSR markers for estimating genetic diversity of horse breeds |
| |
| Authors: | V N Voronkova Tsendsuren Tsedev G E Sulimova |
| |
| Institution: | (1) Plant Molecular Biology Group, Division of Biochemical Sciences, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411 008, India;(2) Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, 411043, India;(3) AICRP on Linseed, College of Agriculture, Nagpur, 440 001, India; |
| |
| Abstract: | The informativeness of six different types of ISSR markers was compared in order to evaluate genetic diversity of Mongolian,
Buryat and Tuvinian horse breeds (Equus caballus). The dinucleotide-based ISSR primers ((GA)9C, (AG)9C, and (CA)9G) proved to be of little use to examine genetic polymorphism among horses due to the low number of amplified and polymorphic
fragments. The polymorphism information content (PIC) for the dinucleotide-based ISSR primers was 2.88 times lower than that
for trinucleotide-based ISSR markers. Among the trinucleotide-based ISSR markers, CAC ISSR marker was also found to be noninformative,
due to the low number of polymorphic loci identified. The highest values of the polymorphism information content (PIC) were
obtained for two types of ISSR markers, based on (GAG)6C (PIC, 3.61) and on (ACC)6G (PIC, 2.50). Thus, the results of the present study showed that GAG and ACC ISSR markers were most informative for evaluation
of genetic diversity among horses. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
