Dietary Excess Vanadium Induces Lesions and Changes of Cell Cycle of Spleen in Broilers |
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Authors: | Wei Cui Hengmin Cui Xi Peng Jing Fang Zhicai Zuo Xiaodong Liu Bangyuan Wu |
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Institution: | (1) Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, 625014, China; |
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Abstract: | The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on spleen growth and lesions by
determining morphological changes and cell cycle of spleen. Four hundred twenty 1-day-old avian broilers were divided into
six groups and fed on a corn–soybean basal diet as control diet or the same diet amended to contain 5, 15, 30, 45, 60 ppm
of vanadium supplied as ammonium metavanadate. When compared with that of control group, the relative weight of spleen was
significantly raised in 5- and 15-ppm groups, but depressed in 45- and 60-ppm groups. The gross lesions of spleen showed obvious
atrophy with decreased volume and pale color in 45- and 60-ppm groups. Histopathologically, lymphocytes in splenic corpuscle
and periarterial lymphatic sheath were variously decreased in number in 30-, 45-, and 60-ppm groups. The percentage of static
phase (G0/G1) was significantly decreased, and the percentage of synthesis period (S) phase and the proliferating index (PI) were significantly
increased in 5- and 15-ppm groups. The percentage of G0/G1 phase was significantly increased, and the percentage of mitotic phase (G2 + M), S phase, and PI significantly decreased in 45- and 60-ppm groups. These results suggested that dietary excess vanadium
(45 and 60 ppm) could inhibit growth of spleen and induce lesions in spleen in chicken. |
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